goat anti rat il 6mab Search Results


goat anti rat il 6mab  (R&D Systems)
94
R&D Systems goat anti rat il 6mab
Goat Anti Rat Il 6mab, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/goat+anti+rat+il+6mab/Goat+Anti-Rat+IgG+Antibody/pmc08716408-34-6-8
Average 94 stars, based on 1 article reviews
goat anti rat il 6mab - by Bioz Stars, 2026-09
94/100 stars
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biotinylated goat anti human il 6 mab  (R&D Systems)
93
R&D Systems biotinylated goat anti human il 6 mab
Characterization of the detoxified vaccine antigen hIL-1b(D145K). ( a ) hIL-1RI binding. Serial dilutions of wild-type hIL-1β or hIL-1b(D145K) were mixed with a constant amount of 1 nmol/l <t>biotinylated</t> hIL-1β and applied to ELISA plates that had been coated with hIL-1RI. Obtained OD values were reciprocally transformed to express % receptor binding of wild-type hIL-1β or hIL-1b(D145K), respectively. ( b ) Formation of the ternary hIL-1RI-hIL-1β-hIL-1RAcP signaling complex. Wild-type human IL-1β (0.4 µg/ml) or hIL-1b(D145K) (100 µg/ml) were incubated with hIL-1RAcP (1 µg/ml) and applied to ELISA plates that had been coated with 1 µg/ml of human IL-1RI. Formation of the ternary complex was detected with a hIL-1RAcP-specific antibody. ( c ) hIL-1RII binding. Experimental conditions were as in a but with hIL-1RII coated on the ELISA plate. ( d ) IL-6 secretion. HeLa cells were incubated with serial dilutions of wild-type hIL-1β or hIL-1b (D145K). After an incubation of 4 hours, IL-6 was quantified in supernatants by Sandwich ELISA. ( e ) Cytopathic effect. A375 cells were incubated with serial dilutions of either wild-type hIL-1β or hIL-1b(D145K). After 7 days, viable adherent cells were stained with crystal violet and quantified by measuring optical densities at 600 nm. Shown are mean values from triplicate measurements ± SEM. ( f ) In vivo inflammatory activity. Groups of female C57BL/6 mice ( n = 4) were injected i.p. with 1 µg of either wild-type hIL-1β or hIL-1b(D145K) or s.c. with 25 µg of the conjugate vaccine Qβ-hIL-1b(D145K). Three hours after injection, sera were collected, and IL-6 levels were quantified with a Quantikine ELISA kit (* P < 0.0001).
Biotinylated Goat Anti Human Il 6 Mab, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/goat+anti+rat+il+6mab/Human+IL-4+Biotinylated+Antibody/pmc04362373-180-7-12
Average 93 stars, based on 1 article reviews
biotinylated goat anti human il 6 mab - by Bioz Stars, 2026-09
93/100 stars
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human epcam/trop-1 antibody  (Bio-Techne corporation)
99
Bio-Techne corporation human epcam/trop-1 antibody
Characterization of the detoxified vaccine antigen hIL-1b(D145K). ( a ) hIL-1RI binding. Serial dilutions of wild-type hIL-1β or hIL-1b(D145K) were mixed with a constant amount of 1 nmol/l <t>biotinylated</t> hIL-1β and applied to ELISA plates that had been coated with hIL-1RI. Obtained OD values were reciprocally transformed to express % receptor binding of wild-type hIL-1β or hIL-1b(D145K), respectively. ( b ) Formation of the ternary hIL-1RI-hIL-1β-hIL-1RAcP signaling complex. Wild-type human IL-1β (0.4 µg/ml) or hIL-1b(D145K) (100 µg/ml) were incubated with hIL-1RAcP (1 µg/ml) and applied to ELISA plates that had been coated with 1 µg/ml of human IL-1RI. Formation of the ternary complex was detected with a hIL-1RAcP-specific antibody. ( c ) hIL-1RII binding. Experimental conditions were as in a but with hIL-1RII coated on the ELISA plate. ( d ) IL-6 secretion. HeLa cells were incubated with serial dilutions of wild-type hIL-1β or hIL-1b (D145K). After an incubation of 4 hours, IL-6 was quantified in supernatants by Sandwich ELISA. ( e ) Cytopathic effect. A375 cells were incubated with serial dilutions of either wild-type hIL-1β or hIL-1b(D145K). After 7 days, viable adherent cells were stained with crystal violet and quantified by measuring optical densities at 600 nm. Shown are mean values from triplicate measurements ± SEM. ( f ) In vivo inflammatory activity. Groups of female C57BL/6 mice ( n = 4) were injected i.p. with 1 µg of either wild-type hIL-1β or hIL-1b(D145K) or s.c. with 25 µg of the conjugate vaccine Qβ-hIL-1b(D145K). Three hours after injection, sera were collected, and IL-6 levels were quantified with a Quantikine ELISA kit (* P < 0.0001).
Human Epcam/Trop 1 Antibody, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/goat+anti+rat+il+6mab/Human+EpCAM%2FTROP-1+Antibody/custom%40af960%4026053043
Average 99 stars, based on 1 article reviews
human epcam/trop-1 antibody - by Bioz Stars, 2026-09
99/100 stars
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alexa fluor 647 conjugated goat anti mouse igg pabs  (Jackson Immuno)
96
Jackson Immuno alexa fluor 647 conjugated goat anti mouse igg pabs
Characterization of the detoxified vaccine antigen hIL-1b(D145K). ( a ) hIL-1RI binding. Serial dilutions of wild-type hIL-1β or hIL-1b(D145K) were mixed with a constant amount of 1 nmol/l <t>biotinylated</t> hIL-1β and applied to ELISA plates that had been coated with hIL-1RI. Obtained OD values were reciprocally transformed to express % receptor binding of wild-type hIL-1β or hIL-1b(D145K), respectively. ( b ) Formation of the ternary hIL-1RI-hIL-1β-hIL-1RAcP signaling complex. Wild-type human IL-1β (0.4 µg/ml) or hIL-1b(D145K) (100 µg/ml) were incubated with hIL-1RAcP (1 µg/ml) and applied to ELISA plates that had been coated with 1 µg/ml of human IL-1RI. Formation of the ternary complex was detected with a hIL-1RAcP-specific antibody. ( c ) hIL-1RII binding. Experimental conditions were as in a but with hIL-1RII coated on the ELISA plate. ( d ) IL-6 secretion. HeLa cells were incubated with serial dilutions of wild-type hIL-1β or hIL-1b (D145K). After an incubation of 4 hours, IL-6 was quantified in supernatants by Sandwich ELISA. ( e ) Cytopathic effect. A375 cells were incubated with serial dilutions of either wild-type hIL-1β or hIL-1b(D145K). After 7 days, viable adherent cells were stained with crystal violet and quantified by measuring optical densities at 600 nm. Shown are mean values from triplicate measurements ± SEM. ( f ) In vivo inflammatory activity. Groups of female C57BL/6 mice ( n = 4) were injected i.p. with 1 µg of either wild-type hIL-1β or hIL-1b(D145K) or s.c. with 25 µg of the conjugate vaccine Qβ-hIL-1b(D145K). Three hours after injection, sera were collected, and IL-6 levels were quantified with a Quantikine ELISA kit (* P < 0.0001).
Alexa Fluor 647 Conjugated Goat Anti Mouse Igg Pabs, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/goat+anti+rat+il+6mab/Goat+Anti-Mouse+IgG/pmc06125214-75-58-65
Average 96 stars, based on 1 article reviews
alexa fluor 647 conjugated goat anti mouse igg pabs - by Bioz Stars, 2026-09
96/100 stars
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his 6 mab antibody  (GenScript corporation)
90
GenScript corporation his 6 mab antibody
Characterization of the detoxified vaccine antigen hIL-1b(D145K). ( a ) hIL-1RI binding. Serial dilutions of wild-type hIL-1β or hIL-1b(D145K) were mixed with a constant amount of 1 nmol/l <t>biotinylated</t> hIL-1β and applied to ELISA plates that had been coated with hIL-1RI. Obtained OD values were reciprocally transformed to express % receptor binding of wild-type hIL-1β or hIL-1b(D145K), respectively. ( b ) Formation of the ternary hIL-1RI-hIL-1β-hIL-1RAcP signaling complex. Wild-type human IL-1β (0.4 µg/ml) or hIL-1b(D145K) (100 µg/ml) were incubated with hIL-1RAcP (1 µg/ml) and applied to ELISA plates that had been coated with 1 µg/ml of human IL-1RI. Formation of the ternary complex was detected with a hIL-1RAcP-specific antibody. ( c ) hIL-1RII binding. Experimental conditions were as in a but with hIL-1RII coated on the ELISA plate. ( d ) IL-6 secretion. HeLa cells were incubated with serial dilutions of wild-type hIL-1β or hIL-1b (D145K). After an incubation of 4 hours, IL-6 was quantified in supernatants by Sandwich ELISA. ( e ) Cytopathic effect. A375 cells were incubated with serial dilutions of either wild-type hIL-1β or hIL-1b(D145K). After 7 days, viable adherent cells were stained with crystal violet and quantified by measuring optical densities at 600 nm. Shown are mean values from triplicate measurements ± SEM. ( f ) In vivo inflammatory activity. Groups of female C57BL/6 mice ( n = 4) were injected i.p. with 1 µg of either wild-type hIL-1β or hIL-1b(D145K) or s.c. with 25 µg of the conjugate vaccine Qβ-hIL-1b(D145K). Three hours after injection, sera were collected, and IL-6 levels were quantified with a Quantikine ELISA kit (* P < 0.0001).
His 6 Mab Antibody, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/goat+anti+rat+il+6mab/mabs+antibody/pmc04140675-80-8-10
Average 90 stars, based on 1 article reviews
his 6 mab antibody - by Bioz Stars, 2026-09
90/100 stars
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starbright blue 520 goat anti mouse igg  (Bio-Rad)
95
Bio-Rad starbright blue 520 goat anti mouse igg
Characterization of the detoxified vaccine antigen hIL-1b(D145K). ( a ) hIL-1RI binding. Serial dilutions of wild-type hIL-1β or hIL-1b(D145K) were mixed with a constant amount of 1 nmol/l <t>biotinylated</t> hIL-1β and applied to ELISA plates that had been coated with hIL-1RI. Obtained OD values were reciprocally transformed to express % receptor binding of wild-type hIL-1β or hIL-1b(D145K), respectively. ( b ) Formation of the ternary hIL-1RI-hIL-1β-hIL-1RAcP signaling complex. Wild-type human IL-1β (0.4 µg/ml) or hIL-1b(D145K) (100 µg/ml) were incubated with hIL-1RAcP (1 µg/ml) and applied to ELISA plates that had been coated with 1 µg/ml of human IL-1RI. Formation of the ternary complex was detected with a hIL-1RAcP-specific antibody. ( c ) hIL-1RII binding. Experimental conditions were as in a but with hIL-1RII coated on the ELISA plate. ( d ) IL-6 secretion. HeLa cells were incubated with serial dilutions of wild-type hIL-1β or hIL-1b (D145K). After an incubation of 4 hours, IL-6 was quantified in supernatants by Sandwich ELISA. ( e ) Cytopathic effect. A375 cells were incubated with serial dilutions of either wild-type hIL-1β or hIL-1b(D145K). After 7 days, viable adherent cells were stained with crystal violet and quantified by measuring optical densities at 600 nm. Shown are mean values from triplicate measurements ± SEM. ( f ) In vivo inflammatory activity. Groups of female C57BL/6 mice ( n = 4) were injected i.p. with 1 µg of either wild-type hIL-1β or hIL-1b(D145K) or s.c. with 25 µg of the conjugate vaccine Qβ-hIL-1b(D145K). Three hours after injection, sera were collected, and IL-6 levels were quantified with a Quantikine ELISA kit (* P < 0.0001).
Starbright Blue 520 Goat Anti Mouse Igg, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/goat+anti+rat+il+6mab/StarBright+Blue+520+Goat+Anti-Mouse+IgG/pm41572432-243-22-28
Average 95 stars, based on 1 article reviews
starbright blue 520 goat anti mouse igg - by Bioz Stars, 2026-09
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goat anti rabbit igg alexa fluor 594  (Cell Signaling Technology Inc)
96
Cell Signaling Technology Inc goat anti rabbit igg alexa fluor 594
Characterization of the detoxified vaccine antigen hIL-1b(D145K). ( a ) hIL-1RI binding. Serial dilutions of wild-type hIL-1β or hIL-1b(D145K) were mixed with a constant amount of 1 nmol/l <t>biotinylated</t> hIL-1β and applied to ELISA plates that had been coated with hIL-1RI. Obtained OD values were reciprocally transformed to express % receptor binding of wild-type hIL-1β or hIL-1b(D145K), respectively. ( b ) Formation of the ternary hIL-1RI-hIL-1β-hIL-1RAcP signaling complex. Wild-type human IL-1β (0.4 µg/ml) or hIL-1b(D145K) (100 µg/ml) were incubated with hIL-1RAcP (1 µg/ml) and applied to ELISA plates that had been coated with 1 µg/ml of human IL-1RI. Formation of the ternary complex was detected with a hIL-1RAcP-specific antibody. ( c ) hIL-1RII binding. Experimental conditions were as in a but with hIL-1RII coated on the ELISA plate. ( d ) IL-6 secretion. HeLa cells were incubated with serial dilutions of wild-type hIL-1β or hIL-1b (D145K). After an incubation of 4 hours, IL-6 was quantified in supernatants by Sandwich ELISA. ( e ) Cytopathic effect. A375 cells were incubated with serial dilutions of either wild-type hIL-1β or hIL-1b(D145K). After 7 days, viable adherent cells were stained with crystal violet and quantified by measuring optical densities at 600 nm. Shown are mean values from triplicate measurements ± SEM. ( f ) In vivo inflammatory activity. Groups of female C57BL/6 mice ( n = 4) were injected i.p. with 1 µg of either wild-type hIL-1β or hIL-1b(D145K) or s.c. with 25 µg of the conjugate vaccine Qβ-hIL-1b(D145K). Three hours after injection, sera were collected, and IL-6 levels were quantified with a Quantikine ELISA kit (* P < 0.0001).
Goat Anti Rabbit Igg Alexa Fluor 594, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/goat+anti+rat+il+6mab/Anti-rabbit+IgG+(H%2BL)%2C+F(ab')+2+Fragment/pm30024985-91-15-21
Average 96 stars, based on 1 article reviews
goat anti rabbit igg alexa fluor 594 - by Bioz Stars, 2026-09
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facscan flow cytometer  (Becton Dickinson)
90
Becton Dickinson facscan flow cytometer
Characterization of the detoxified vaccine antigen hIL-1b(D145K). ( a ) hIL-1RI binding. Serial dilutions of wild-type hIL-1β or hIL-1b(D145K) were mixed with a constant amount of 1 nmol/l <t>biotinylated</t> hIL-1β and applied to ELISA plates that had been coated with hIL-1RI. Obtained OD values were reciprocally transformed to express % receptor binding of wild-type hIL-1β or hIL-1b(D145K), respectively. ( b ) Formation of the ternary hIL-1RI-hIL-1β-hIL-1RAcP signaling complex. Wild-type human IL-1β (0.4 µg/ml) or hIL-1b(D145K) (100 µg/ml) were incubated with hIL-1RAcP (1 µg/ml) and applied to ELISA plates that had been coated with 1 µg/ml of human IL-1RI. Formation of the ternary complex was detected with a hIL-1RAcP-specific antibody. ( c ) hIL-1RII binding. Experimental conditions were as in a but with hIL-1RII coated on the ELISA plate. ( d ) IL-6 secretion. HeLa cells were incubated with serial dilutions of wild-type hIL-1β or hIL-1b (D145K). After an incubation of 4 hours, IL-6 was quantified in supernatants by Sandwich ELISA. ( e ) Cytopathic effect. A375 cells were incubated with serial dilutions of either wild-type hIL-1β or hIL-1b(D145K). After 7 days, viable adherent cells were stained with crystal violet and quantified by measuring optical densities at 600 nm. Shown are mean values from triplicate measurements ± SEM. ( f ) In vivo inflammatory activity. Groups of female C57BL/6 mice ( n = 4) were injected i.p. with 1 µg of either wild-type hIL-1β or hIL-1b(D145K) or s.c. with 25 µg of the conjugate vaccine Qβ-hIL-1b(D145K). Three hours after injection, sera were collected, and IL-6 levels were quantified with a Quantikine ELISA kit (* P < 0.0001).
Facscan Flow Cytometer, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/goat+anti+rat+il+6mab/facscalibur+flow+cytometer/pmc02947239-307-21-28
Average 90 stars, based on 1 article reviews
facscan flow cytometer - by Bioz Stars, 2026-09
90/100 stars
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cyanine dye 3 conjugated donkey anti goat igg  (Jackson Immuno)
96
Jackson Immuno cyanine dye 3 conjugated donkey anti goat igg
Characterization of the detoxified vaccine antigen hIL-1b(D145K). ( a ) hIL-1RI binding. Serial dilutions of wild-type hIL-1β or hIL-1b(D145K) were mixed with a constant amount of 1 nmol/l <t>biotinylated</t> hIL-1β and applied to ELISA plates that had been coated with hIL-1RI. Obtained OD values were reciprocally transformed to express % receptor binding of wild-type hIL-1β or hIL-1b(D145K), respectively. ( b ) Formation of the ternary hIL-1RI-hIL-1β-hIL-1RAcP signaling complex. Wild-type human IL-1β (0.4 µg/ml) or hIL-1b(D145K) (100 µg/ml) were incubated with hIL-1RAcP (1 µg/ml) and applied to ELISA plates that had been coated with 1 µg/ml of human IL-1RI. Formation of the ternary complex was detected with a hIL-1RAcP-specific antibody. ( c ) hIL-1RII binding. Experimental conditions were as in a but with hIL-1RII coated on the ELISA plate. ( d ) IL-6 secretion. HeLa cells were incubated with serial dilutions of wild-type hIL-1β or hIL-1b (D145K). After an incubation of 4 hours, IL-6 was quantified in supernatants by Sandwich ELISA. ( e ) Cytopathic effect. A375 cells were incubated with serial dilutions of either wild-type hIL-1β or hIL-1b(D145K). After 7 days, viable adherent cells were stained with crystal violet and quantified by measuring optical densities at 600 nm. Shown are mean values from triplicate measurements ± SEM. ( f ) In vivo inflammatory activity. Groups of female C57BL/6 mice ( n = 4) were injected i.p. with 1 µg of either wild-type hIL-1β or hIL-1b(D145K) or s.c. with 25 µg of the conjugate vaccine Qβ-hIL-1b(D145K). Three hours after injection, sera were collected, and IL-6 levels were quantified with a Quantikine ELISA kit (* P < 0.0001).
Cyanine Dye 3 Conjugated Donkey Anti Goat Igg, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/goat+anti+rat+il+6mab/Donkey+Anti-Goat+IgG/pmc07019028-46-103-122
Average 96 stars, based on 1 article reviews
cyanine dye 3 conjugated donkey anti goat igg - by Bioz Stars, 2026-09
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human/primate il-6 antibody  (Bio-Techne corporation)
95
Bio-Techne corporation human/primate il-6 antibody
Characterization of the detoxified vaccine antigen hIL-1b(D145K). ( a ) hIL-1RI binding. Serial dilutions of wild-type hIL-1β or hIL-1b(D145K) were mixed with a constant amount of 1 nmol/l <t>biotinylated</t> hIL-1β and applied to ELISA plates that had been coated with hIL-1RI. Obtained OD values were reciprocally transformed to express % receptor binding of wild-type hIL-1β or hIL-1b(D145K), respectively. ( b ) Formation of the ternary hIL-1RI-hIL-1β-hIL-1RAcP signaling complex. Wild-type human IL-1β (0.4 µg/ml) or hIL-1b(D145K) (100 µg/ml) were incubated with hIL-1RAcP (1 µg/ml) and applied to ELISA plates that had been coated with 1 µg/ml of human IL-1RI. Formation of the ternary complex was detected with a hIL-1RAcP-specific antibody. ( c ) hIL-1RII binding. Experimental conditions were as in a but with hIL-1RII coated on the ELISA plate. ( d ) IL-6 secretion. HeLa cells were incubated with serial dilutions of wild-type hIL-1β or hIL-1b (D145K). After an incubation of 4 hours, IL-6 was quantified in supernatants by Sandwich ELISA. ( e ) Cytopathic effect. A375 cells were incubated with serial dilutions of either wild-type hIL-1β or hIL-1b(D145K). After 7 days, viable adherent cells were stained with crystal violet and quantified by measuring optical densities at 600 nm. Shown are mean values from triplicate measurements ± SEM. ( f ) In vivo inflammatory activity. Groups of female C57BL/6 mice ( n = 4) were injected i.p. with 1 µg of either wild-type hIL-1β or hIL-1b(D145K) or s.c. with 25 µg of the conjugate vaccine Qβ-hIL-1b(D145K). Three hours after injection, sera were collected, and IL-6 levels were quantified with a Quantikine ELISA kit (* P < 0.0001).
Human/Primate Il 6 Antibody, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/goat+anti+rat+il+6mab/Human%2FPrimate+IL-6+Antibody/custom%40mab206%4010861088
Average 95 stars, based on 1 article reviews
human/primate il-6 antibody - by Bioz Stars, 2026-09
95/100 stars
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rat anti-human integrin 6 mab  (Immunotec inc)
90
Immunotec inc rat anti-human integrin 6 mab
Characterization of the detoxified vaccine antigen hIL-1b(D145K). ( a ) hIL-1RI binding. Serial dilutions of wild-type hIL-1β or hIL-1b(D145K) were mixed with a constant amount of 1 nmol/l <t>biotinylated</t> hIL-1β and applied to ELISA plates that had been coated with hIL-1RI. Obtained OD values were reciprocally transformed to express % receptor binding of wild-type hIL-1β or hIL-1b(D145K), respectively. ( b ) Formation of the ternary hIL-1RI-hIL-1β-hIL-1RAcP signaling complex. Wild-type human IL-1β (0.4 µg/ml) or hIL-1b(D145K) (100 µg/ml) were incubated with hIL-1RAcP (1 µg/ml) and applied to ELISA plates that had been coated with 1 µg/ml of human IL-1RI. Formation of the ternary complex was detected with a hIL-1RAcP-specific antibody. ( c ) hIL-1RII binding. Experimental conditions were as in a but with hIL-1RII coated on the ELISA plate. ( d ) IL-6 secretion. HeLa cells were incubated with serial dilutions of wild-type hIL-1β or hIL-1b (D145K). After an incubation of 4 hours, IL-6 was quantified in supernatants by Sandwich ELISA. ( e ) Cytopathic effect. A375 cells were incubated with serial dilutions of either wild-type hIL-1β or hIL-1b(D145K). After 7 days, viable adherent cells were stained with crystal violet and quantified by measuring optical densities at 600 nm. Shown are mean values from triplicate measurements ± SEM. ( f ) In vivo inflammatory activity. Groups of female C57BL/6 mice ( n = 4) were injected i.p. with 1 µg of either wild-type hIL-1β or hIL-1b(D145K) or s.c. with 25 µg of the conjugate vaccine Qβ-hIL-1b(D145K). Three hours after injection, sera were collected, and IL-6 levels were quantified with a Quantikine ELISA kit (* P < 0.0001).
Rat Anti Human Integrin 6 Mab, supplied by Immunotec inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/goat+anti+rat+il+6mab/goh3/10__1074_slash_jbc__m109__082875-88-26-37
Average 90 stars, based on 1 article reviews
rat anti-human integrin 6 mab - by Bioz Stars, 2026-09
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mouse anti-human mrp2 mab  (Enzo Biochem)
90
Enzo Biochem mouse anti-human mrp2 mab
Characterization of the detoxified vaccine antigen hIL-1b(D145K). ( a ) hIL-1RI binding. Serial dilutions of wild-type hIL-1β or hIL-1b(D145K) were mixed with a constant amount of 1 nmol/l <t>biotinylated</t> hIL-1β and applied to ELISA plates that had been coated with hIL-1RI. Obtained OD values were reciprocally transformed to express % receptor binding of wild-type hIL-1β or hIL-1b(D145K), respectively. ( b ) Formation of the ternary hIL-1RI-hIL-1β-hIL-1RAcP signaling complex. Wild-type human IL-1β (0.4 µg/ml) or hIL-1b(D145K) (100 µg/ml) were incubated with hIL-1RAcP (1 µg/ml) and applied to ELISA plates that had been coated with 1 µg/ml of human IL-1RI. Formation of the ternary complex was detected with a hIL-1RAcP-specific antibody. ( c ) hIL-1RII binding. Experimental conditions were as in a but with hIL-1RII coated on the ELISA plate. ( d ) IL-6 secretion. HeLa cells were incubated with serial dilutions of wild-type hIL-1β or hIL-1b (D145K). After an incubation of 4 hours, IL-6 was quantified in supernatants by Sandwich ELISA. ( e ) Cytopathic effect. A375 cells were incubated with serial dilutions of either wild-type hIL-1β or hIL-1b(D145K). After 7 days, viable adherent cells were stained with crystal violet and quantified by measuring optical densities at 600 nm. Shown are mean values from triplicate measurements ± SEM. ( f ) In vivo inflammatory activity. Groups of female C57BL/6 mice ( n = 4) were injected i.p. with 1 µg of either wild-type hIL-1β or hIL-1b(D145K) or s.c. with 25 µg of the conjugate vaccine Qβ-hIL-1b(D145K). Three hours after injection, sera were collected, and IL-6 levels were quantified with a Quantikine ELISA kit (* P < 0.0001).
Mouse Anti Human Mrp2 Mab, supplied by Enzo Biochem, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/goat+anti+rat+il+6mab/monoclonal+antibody+human+mrp2/pmc02720231-69-62-68
Average 90 stars, based on 1 article reviews
mouse anti-human mrp2 mab - by Bioz Stars, 2026-09
90/100 stars
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Characterization of the detoxified vaccine antigen hIL-1b(D145K). ( a ) hIL-1RI binding. Serial dilutions of wild-type hIL-1β or hIL-1b(D145K) were mixed with a constant amount of 1 nmol/l biotinylated hIL-1β and applied to ELISA plates that had been coated with hIL-1RI. Obtained OD values were reciprocally transformed to express % receptor binding of wild-type hIL-1β or hIL-1b(D145K), respectively. ( b ) Formation of the ternary hIL-1RI-hIL-1β-hIL-1RAcP signaling complex. Wild-type human IL-1β (0.4 µg/ml) or hIL-1b(D145K) (100 µg/ml) were incubated with hIL-1RAcP (1 µg/ml) and applied to ELISA plates that had been coated with 1 µg/ml of human IL-1RI. Formation of the ternary complex was detected with a hIL-1RAcP-specific antibody. ( c ) hIL-1RII binding. Experimental conditions were as in a but with hIL-1RII coated on the ELISA plate. ( d ) IL-6 secretion. HeLa cells were incubated with serial dilutions of wild-type hIL-1β or hIL-1b (D145K). After an incubation of 4 hours, IL-6 was quantified in supernatants by Sandwich ELISA. ( e ) Cytopathic effect. A375 cells were incubated with serial dilutions of either wild-type hIL-1β or hIL-1b(D145K). After 7 days, viable adherent cells were stained with crystal violet and quantified by measuring optical densities at 600 nm. Shown are mean values from triplicate measurements ± SEM. ( f ) In vivo inflammatory activity. Groups of female C57BL/6 mice ( n = 4) were injected i.p. with 1 µg of either wild-type hIL-1β or hIL-1b(D145K) or s.c. with 25 µg of the conjugate vaccine Qβ-hIL-1b(D145K). Three hours after injection, sera were collected, and IL-6 levels were quantified with a Quantikine ELISA kit (* P < 0.0001).

Journal: Molecular Therapy. Methods & Clinical Development

Article Title: Preclinical efficacy and safety of an anti-IL-1β vaccine for the treatment of type 2 diabetes

doi: 10.1038/mtm.2014.48

Figure Lengend Snippet: Characterization of the detoxified vaccine antigen hIL-1b(D145K). ( a ) hIL-1RI binding. Serial dilutions of wild-type hIL-1β or hIL-1b(D145K) were mixed with a constant amount of 1 nmol/l biotinylated hIL-1β and applied to ELISA plates that had been coated with hIL-1RI. Obtained OD values were reciprocally transformed to express % receptor binding of wild-type hIL-1β or hIL-1b(D145K), respectively. ( b ) Formation of the ternary hIL-1RI-hIL-1β-hIL-1RAcP signaling complex. Wild-type human IL-1β (0.4 µg/ml) or hIL-1b(D145K) (100 µg/ml) were incubated with hIL-1RAcP (1 µg/ml) and applied to ELISA plates that had been coated with 1 µg/ml of human IL-1RI. Formation of the ternary complex was detected with a hIL-1RAcP-specific antibody. ( c ) hIL-1RII binding. Experimental conditions were as in a but with hIL-1RII coated on the ELISA plate. ( d ) IL-6 secretion. HeLa cells were incubated with serial dilutions of wild-type hIL-1β or hIL-1b (D145K). After an incubation of 4 hours, IL-6 was quantified in supernatants by Sandwich ELISA. ( e ) Cytopathic effect. A375 cells were incubated with serial dilutions of either wild-type hIL-1β or hIL-1b(D145K). After 7 days, viable adherent cells were stained with crystal violet and quantified by measuring optical densities at 600 nm. Shown are mean values from triplicate measurements ± SEM. ( f ) In vivo inflammatory activity. Groups of female C57BL/6 mice ( n = 4) were injected i.p. with 1 µg of either wild-type hIL-1β or hIL-1b(D145K) or s.c. with 25 µg of the conjugate vaccine Qβ-hIL-1b(D145K). Three hours after injection, sera were collected, and IL-6 levels were quantified with a Quantikine ELISA kit (* P < 0.0001).

Article Snippet: Bound human IL-6 was detected with a biotinylated goat anti-human IL-6 mAb (R&D Systems) and horseradish peroxidase-conjugated streptavidin.

Techniques: Binding Assay, Enzyme-linked Immunosorbent Assay, Transformation Assay, Incubation, Sandwich ELISA, Staining, In Vivo, Activity Assay, Injection